T7

Part:BBa_K5250003:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-09-28)


PT7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7-like promoter can prove difficult to clone due to its small size.


Source

Sequence is derived from a Pseudomonas phage Pf-10 gene bank: https://ncbi.nlm.nih.gov/datasets/genome/GCF_001038595.1/

References

Lammens, E., Feyaerts, N., Kerremans, A., Boon, M., & Lavigne, R. (2023). Assessing the orthogonality of Phage-Encoded RNA polymerases for tailored synthetic biology applications in pseudomonas species. International Journal of Molecular Sciences, 24(8), 7175. https://doi.org/10.3390/ijms24087175